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NALIA Technology

Cost-effective, rapid multiplex immunodiagnostics

NALIA's technology retains all the benefits of traditional ELISA tests, but in multiplex form, offering huge cost savings.

A NALIA Array is based on the same plate format as the common ELISA test, offering all the same benefits of sensitivity, accuracy, discrimination and quantification, but uniquely uses membrane-bottomed wells, on the underside of which is an array of detection spots.

Initial NALIA Array products will use a 5x5 array of spots in each well, testing 10 different biomarkers, each in duplicate plus internal controls. This allows for the testing of more than twice the number of patients for 10 times the number of biomarkers compared to a standard ELISA plate.

NALIA also produces a hexagonal array of 37 spots, allowing for the testing of 16 different biomarkers per sample. NALIA systems is also developing higher density arrays for use in the near future.

The assay is performed by drawing the patient’s blood sample, followed by a series of reagents and wash solutions, across the membrane using a light vacuum. The membrane filters off any solid material so that a pinprick of blood only requires dilution and does not require preparation to remove blood cells. Biomarkers present in the blood sample interact with the multiple detection reagents in the array as they pass through the membrane.

The final step is a light or colour-generating reaction process, the intensity of which is proportionate to the level of the target biomarker in the sample. The plate is photographed and the intensity of reaction at each spot analysed by a software algorithm. For some applications, visual scoring of the intensity of colour spots will be sufficient.

In contrast to competitors’ solutions, which are largely based on genomic and proteomic technology, test very large numbers of markers and require major investment in capital equipment and re-training for users, NALIA’s system can be readily integrated into existing clinical laboratories in both developed and developing countries.